The study was aimed to see the cellular level body composition difference among the two groups of gender. For the purpose of this study the data was collected on the selected cellular level body composition variables body fat, protein, mineral and glycogen percentage. The data was collected on the sportspersons of the LNIPE Gwalior. The non-probability and probability sampling method was applied for female and male respectively to choose out the thirteen samples separately for both the groups from the population of sportspersons. The age of the subjects is ranges between 17-25years. The data was analysed with the help of SPSS22 version by applying statistical technique independent sample t-test. After analysing the raw data it was concluded that the fat percentage is varies between both the groups. The fat percentage for female sportsperson is significantly higher than the male sportsperson at the 0.05 level of significant. Whereas no difference is found among all other variables protein, mineral and glycogen because the statistical p value for these variables is greater than 0.05 which signifies the amount of these cellular level variables are same for both the groups of sportsperson.