Objective: To determine prevalence of vacA, cagA and oipA genotypes in gastric biopsies in Abidjan (Côte d'Ivoire). Material and methods: A total of 98 positive urea rapid-biopsy specimens in an endoscopy room at Hospital and University Center of Cocody (Abidjan) for period from August 2015 to January 2016 were selected for the study. Gastric biopsies were collected and sent to Laboratory of Pasteur Institute of Côte d'Ivoire under conditions and delays of routing. Genes were detected by PCR. Results: Gastric biopsies that had positive PCR to one or more genes were 71.4% (70/98). CagA and oipA genotypes were detected at 72.8% and 37.2%, respectively. VacA s1a region was identified in 15.7% (11/70) and the VacA m1a region in 5.7% (4/70). Taken together, vacA s1a / m1a allele was identified at 55.7% (39/70). 61.5% of patients were women and 38.5% of men with a sex ratio of 0.62. Average age was 41.7 years (between 19 and 72 years). Conclusion: High prevalence of infection by virulent factors could be used to discern risk of developing severe gastroduodenal diseases in host and contribute to characteristics of H. pylori infection.