Nurses are the major health care provider in the hospital and are more potential to get exposed to spillage with blood and body fluid. Previous studies have shown that Nurses are having inadequate knowledge regarding Spillage Management. The purpose of this study was to assess the knowledge level of Staff Nurses regarding Spillage Management. 10 Staff Nurses were selected through the Non probability purposive sampling technique and their knowledge level was assessed by Structured knowledge questionnaire. Mean and standard deviation of knowledge score was calculated in which, the mean value was 9.5 and the standard deviation was 1.301. Chi square was used to find the association of knowledge score with selected socio demographic variables. It was found that the p value was 0.048 and was less than the table value. Thus, the study revealed that there is an association of knowledge scores with selected socio demographic variables.