The present research focuses to prepare novel bioactive compound from two different plant sources using classical biochemical Techniques and formulate a topical product with therapeutic efficiency against UV B induced skin damage. Catechin was purified from Green tea and impregnated in Aloe vera gel and used for topical application to evaluate its efficiency to protect skin against radiation. In the study, these two different bioactive substances were formulated as a single product which could be topically applied to treat skin damage induced by UVB radiation in animal model. The results revealed phytochemicals posses anti-oxidant activity that exerts free radical scavenger role to reduce toxic effect thereby promoting regeneration of epidermal cell growth. Antioxidant activities was decreased in the control batch but was increased in product treated experimental animals indicated Antioxidant activities such as GSH, catalase, SOD,GP,GR showed defense against oxidative stress. Results suggested beneficial effects of topical application of formulated herbal product in UVB induced skin damages. Based on these promising findings, the addition of tea polyphenols into topical products such as sunscreens and creams may enhance protection against the development of UVB-induced skin cancer by reducing the medical and economic burdens of this disease.